Inbred corn line ZS01452

ABSTRACT

Broadly this invention provides inbred corn line ZS01452. The methods for producing a corn plant by crossing the inbred line ZS01452 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01452 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01452 with at least one other corn line.

FIELD OF THE INVENTION

This invention is in the field of corn breeding, specifically relating to an inbred corn line designated ZS01452.

BACKGROUND OF THE INVENTION

The original maize plant was indigenous to the Western Hemisphere. The plants were weedlike and only through the efforts of early breeders was a cultivated crop species developed. The physical traits of maize are such that self pollination or cross pollination can occur. Each plant has a separate male and female flower, the tassel and ear, respectively. Natural pollination occurs when wind transfers pollen from tassel to the silks on the corn ears. This type of pollination contributed to the wide variation of maize varieties present in the Western Hemisphere.

The development of a planned breeding program for maize only occurred in the last century. Originally, maize was an open pollinated variety having heterogeneous genotypes. The maize farmer selected uniform ears from the yield of these genotypes and reserved them for planting the next season. The result was a field of maize plants that were segregating for a variety of traits. This type of maize selection lead to at most incremental increases in seed yield.

Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced.

The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance.

Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F₂ population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection.

Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc.

To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F₁, generation and self pollinating to produce a F₂ generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids.

SUMMARY OF THE INVENTION

The present invention relates to an inbred corn line ZS01452. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01452.

Generally then, broadly the present invention includes an inbred corn seed designated ZS01452. This seed produces a corn plant.

The invention also includes the tissue culture of regenerable cells of ZS01452 wherein the tissue regenerates plants having the genotype of ZS01452. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01452 having ZS01452's genotype.

The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01452 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01452 and plants of another inbred line. Hybrid plants grown from this hybrid seed.

The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01452 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed.

A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01452 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above.

DEFINITIONS

In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided.

BL MOIST

The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity.

COLD GERM

Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating.

ECB

European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers.

EMERGE

The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves.

GI

This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula:

    GI=100+0.5(YLD)-0.9(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR)

GLS

Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*

GW

Goss' Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*

HEATP10

The number of Growing Degree Units (GDU's) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU's for a 24 hour period are: ##EQU1## The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDU's to reach various stages of plant development.

HEATBL

The number of GDU's after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer).

HEATPEEK

The number of GDU's after planting of an inbred when approximately 50 percent of the plants show visible tassel extension.

HEATP50 or HTP50

The number of GDU's required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.

HEATP90

The number of GDU's accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.

HEATS10

The number of GDU's required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.

HEATS50 or HTS50

The number of GDU's required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches, Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.

HEATS90

The number of GDU's required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition.

MDMV_(A)

Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*

MDMV_(B)

Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a "1" being very susceptible and a "9" being very resistant.*

MOISTURE

The average percentage grain moisture of an inbred or hybrid at harvest time.

NLB

Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*

PCT TILLER

The total number of tillers per plot divided by the total number of plants per plot.

PLANT

This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like.

PLANT HEIGHT

The distance in centimeters from ground level to the base of the tassel peduncle.

RM

Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System.

SHED

The volume of pollen shed by the male flower rated on a 1-9 scale where a "1" is a very light pollen shedder, a "4.5" is a moderate shedder, and a "9" is a very heavy shedder. If the Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale then any shed on Table 3 can be multiplied by 3 to reach the 1-9 shed scale.

SLB

Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a "1" being very susceptible, and a "9" being very resistant.*

TWT

The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture.

VIGOR

Visual rating of 1 to 9 made 2-3 weeks post-emergence where a "1" indicates very poor early plant development, and a "9" indicates superior plant development.

WARM GERM

A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating.

YIELD (YLD)

Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre.

% DROPPED EARS (DE)

The number of plants per plot which dropped their primary ear divided by the total number of plants per plot.

% LRG FLAT

Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings.

% LRG ROUND

Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings.

% MED FLAT

Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings.

% MED ROUND

Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings.

% SML FLAT

Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings.

% SML ROUND

Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings.

% ROOT LODGE (RL)

Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot.

% STALK LODGE (SL)

Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot.

DETAILED DESCRIPTION OF THE INVENTION

ZS01452 can be used as a male line due to its profuse pollen shed. This ZS01452 line evidences good general combining ability with stiff stalk females. This line makes attractive hybrids, with upright ears, and keeps the taps on the hybrids at harvest.

The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Slater, Iowa.

The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01452.

The best method of producing the invention, ZS01452 which is substantially homozygous, is by planting the seed of ZS01452 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen.

                                      TABLE 1                                      __________________________________________________________________________     ZS01452                                                                        VARIETY DESCRIPTION INFORMATION                                                __________________________________________________________________________     #1  Type: Dent                                                                 #2  Region Best Adapted: Northern and possibly in Michigan and Ontario             regions.                                                                   ON PLANT MORPHOLOGICAL DESCRIPTION                                             INBRED ZS01452                                                                 #3                                                                             PLANT TRAITS          LEAF TRAITS                                              PLANT HEIGHT 61 IN.   LEAVES ABOVE EAR                                                                             4                                          EAR HEIGHT   29 IN.   LEAVES BELOW EAR                                                                             4                                          BRACE ROOT COLOR                                                                            YELLOW/GREEN                                                                            LEAF ANGLE ABOVE EAR                                                                         SEMI-ERECT                                 SHOOTS AT FLOWERING                                                                         BALD     LEAF ANGLE BELOW EAR                                                                         OPEN                                       SILK COLOR   PALE GREEN                                                                              FLAG LEAF ANGLE                                                                              SEMI-ERECT                                 COB COLOR    PINK     LEAF COLOR    MEDIUM GREEN                               KERNEL ROWS  14       LEAF MARGIN COLOR                                                                            WHITE                                      TASSEL TRAITS         EAR AND KERNEL TRAITS                                    TASSEL SIZE  16 IN.   EAR LENGTH    6 IN.                                      NUMBER OF BRANCHES                                                                          7        EAR DIAMETER  1.4 IN.                                    TASSEL BRANCH ANGLE                                                                         ERECT    COB DIAMETER  IN.                                        GLUME COLOR  GREEN    KERNEL CROWN COLOR                                                                           LIGHT YELLOW                               ANTHER COLOR YELLOW   KERNEL BODY COLOR                                                                            DARK YELLOW                                GLUME RING COLOR                                                                            WHITE                                                             #4  DISEASE RESISTANCE                                                                               Northern leaf blight = 1                                                       NLSr2* = 1                                                                     Gray leaf spot = --                                                            Eye = 1.5                                                                      GW = 2                                                                         MDMVB = 1                                                #5  The present invention can best be compared to PVP# 8700088                 __________________________________________________________________________      *Northern Leaf Spot Race Two                                             

The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding.

The purity and homozygosity of inbred ZS01452 is constantly being tracked using isozyme genotypes as shown in Table 2.

Isozyme Genotypes for ZS01452

Isozyme data were generated for inbred corn line ZS01452 according to procedures known and published in the art. The data in Table 2 gives the electrophoresis data on ZS01452.

                                      TABLE 2                                      __________________________________________________________________________     ELECTROPHORESIS RESULTS FOR ZS01452                                            INBRED                                                                              ACP1                                                                               ACP4                                                                               ADH                                                                               MDH1                                                                               MDH2                                                                               PGD1                                                                               PGD2                                                                               PH1                                                                               PGM                                                                               IDH2                                     __________________________________________________________________________     ZS01452                                                                             11  00  22 22  11  11  11  22 22 11                                       __________________________________________________________________________

Inbred and Hybrid Performance of ZS01452

The traits and characteristics of inbred corn line ZS01452 are listed to compare with other inbreds and/or in hybrid combination. ZS01452 data shows the characteristics and traits of importance, giving a snapshot of ZS01452.

Table 3A compares ZS01452 will PVP#8700088. ZS01452 has significantly better seedling vigor and emergence than PVP#8700088. It is a taller plant and has higher ear height than PVP#8700088. ZS01452 tassel emergence is earlier and the inbred begins to shed pollen earlier than PVP#8700088 as evidenced by the earlier HeatPEEK and HeatP10. ZS01452 also silks significantly earlier than PVP#8700088 as the HeatS10, HeatS50 and HeatS90 ratings indicate. ZS01452 kernels seed size is generally larger than PVP#8700088.

                                      TABLE 3A                                     __________________________________________________________________________     PAIRED INBRED COMPARISON DATA                                                  __________________________________________________________________________                               PCT  PLANT EAR          EAR    PCT                   YEAR  INBRED  VIGOR EMERGE                                                                               TILLER                                                                              HEIGHT                                                                               HEIGHT                                                                              SHED    QUALITY                                                                               BARREN                __________________________________________________________________________     OVERALL                                                                              ZS01452 6.8   80.2       153.7 69.6 5.2                                        PVP#8700088                                                                            5.8   91.7       133.6 49.9 4.0                                        # EXPTS 5     5          5     5    5                                          DIFF    1.0   11.4       20.1  19.6 1.2                                        PROB    0.003*                                                                               0.005*     0.001*                                                                               0.001*                                                                              0.178                                __________________________________________________________________________     YEAR  INBRED  HEATP10                                                                              HEATP50                                                                              HEATP90                                                                             HEATS10                                                                              HEATS50                                                                             HEATS90                              __________________________________________________________________________     OVERALL                                                                              ZS01452 1206  1276  1405 1230  1271 1308                                       PVP#8700088                                                                            1249  1305  1422 1290  1323 1357                                       # EXPTS 5     5     5    5     5    5                                          DIFF    43    30    17   60    52   49                                         PROB    0.078***                                                                             0.289 0.532                                                                               0.000*                                                                               0.014**                                                                             0.065***                             __________________________________________________________________________                               BL   % ROOT                                                                               % STALK                                                                             % DROPPED                            YEAR  INBRED  HEATPEEK                                                                             HEATBL                                                                               MOIST                                                                               LODGE LODGE                                                                               EARS    MOISTURE                                                                              YIELD                 __________________________________________________________________________     OVERALL                                                                              ZS01452 1095  2355                          11.3   69.7                        PVP#8700088                                                                            1124  2343                          11.4   65.8                        # EXPTS 5     1                             5      5                           DIFF    29    12                            0.1    3.9                         PROB    0.056***                            0.947                        __________________________________________________________________________                   WARM  COLD  % LRG                                                                               % LRG % MED                                                                               % MED   % SML  % SML                 YEAR  INBRED  GERM  GERM  ROUND                                                                               FLAT  ROUND                                                                               FLAT    ROUND  FLAT                  __________________________________________________________________________     OVERALL                                                                              ZS01452 90.2  69.9  19.0 7.2   38.0 25.8    10.2   3.4                         PVP#8700088                                                                            95.0  81.3  0.3  6.2   12.8 59.8    7.0    10.8                        # EXPTS 5     5     3    5     5    5       5      5                           DIFF    4.9   11.4  18.7 1.0   25.2 34.0    3.2    7.4                         PROB    0.061***                                                                             0.091***                                                                             0.000*                                                                              0.686 0.000*                                    __________________________________________________________________________

Table 4 shows the GCA (general combining ability) estimates of ZS01452 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of "checks". The check data is from other companies' hybrids, particularly the leader in the industry and ICI Seeds' commercial products and pre-commercial hybrids which were grown in the same sets and locations.

Table 4A shows ZS01452 crossed to 48 different inbreds. In hybrid combination, ZS01452 shows very good performance in all categories except root lodging. The strong yield advantage and moisture and stalk lodging advantage result in very good FI and GI index ratings. The ZS01452 hybrids exhibit early maturity, high yield and good standability.

                                      TABLE 4A                                     __________________________________________________________________________     GCA & SCA ESTIMATES                           ENT                              ADVANTAGES OVER COMMERCIALS & N'S             ZS01452                          N93   N94                                                                               N95                                                                               N  FI                                                                               Y M                                                                               GI I  YLD                                                                               MST                                                                               % SL                                                                              % RL                                                                              % DE                                                                               TWT                                                                               POP                                                                               RM                             __________________________________________________________________________     XR =                                                                              421                                                                               377                                                                               576                                                                               1423                                                                              2.9                                                                              0.8                                                                               -4.2                                                                              -2.7                                                                              -7.0                                                                              3.1                                                                               -0.4                                                                              -0.3                                                                              -0.0                                                                               -1.1                                                                              -150                                                                              108                            XH =        164                                                                               1.6                                                                              0.7                                                                               -6.6                                                                              -4.3                                                                              -11.5                                                                             3.5                                                                               -0.6                                                                              -0.4                                                                              -0.0                                                                               -1.2                                                                              -185                                                                              108                            XT =        37 4.1                                                                              0.8                                                                               -2.0                                                                              -1.0                                                                              -3.2                                                                              2.7                                                                               0.0                                                                               -0.2                                                                              -0.1                                                                               -0.9                                                                              -347                                                                              109                            XR =                                                                              38    9  48 3.4                                                                              0.4                                                                               1.9                                                                               -0.8                                                                              3.0                                                                               0.7                                                                               0.4                                                                               -0.3                                                                              0.1 -0.1                                                                              -54                                                                               104                            XH =        6  6.2                                                                              0.7                                                                               1.1                                                                               -0.8                                                                              -0.3                                                                              2.3                                                                               0.8                                                                               -0.1                                                                              0.2 0.1                                                                               -169                                                                              102                            XT =        1  1.6                                                                              0.1                                                                               2.2                                                                               -0.8                                                                              4.3                                                                               -0.3                                                                              0.1                                                                               -0.5                                                                              0.1 -0.4                                                                              -62                                                                               105                            __________________________________________________________________________      XR = GCA ESTIMATE: WEIGHTED BY EXPT                                            XH = GCA ESTIMATE: WEIGHTED BY PARENT 2                                        XT = SAME AS XH. BUT USING ONLY THOSE PARENT 2 WITH TWO YEARS OF DATA    

Table 4B compares ZS01452 in random hybrid combination with PVP#8700088 in random combinations. ZS01452 has an advantage in most category over PVP#8700088. Yield is must improved compared to PVP#8700088, with a better overall Y/M. Percent stalk lodging and dropped ears have a slight advantage of ZS01452, while there is a slight disadvantage for percent root lodging and test weight. The positive advantages of the FI and GI indexes in favor of ZS01452 indicate the strength of performance of the inbred for its region of adaptation.

                                      TABLE 4B                                     __________________________________________________________________________               N   FI YM GI I  YLD                                                                               MST                                                                               % SL                                                                              % RL                                                                              % DE                                                                               TWT                                                                               POP                                                                               RM                             __________________________________________________________________________     ZS01452                                                                               XR =                                                                              148 3.4                                                                               0.4                                                                               1.9                                                                               -0.8                                                                              3.0                                                                               0.7                                                                               0.4                                                                               -0.3                                                                              0.1 -0.1                                                                              -54                                                                               104                            PVP# 8700088                                                                          XR =                                                                              8471                                                                               -0.1                                                                              0.0                                                                               -3.1                                                                              -2.6                                                                              -6.6                                                                              1.3                                                                               0.0                                                                               0.3                                                                               0.0 0.4                                                                               -31                                                                               92                             __________________________________________________________________________

ZS01452 should be used as a male in a hybrid production field. ZS01452 sheds a satisfactory amount of pollen over an extended period of time. ZS01452's susceptibility to ear rots preclude its use as a female. ZS01452 is a medium tall plant with moderately low ear placement. It flowers early and make hybrids which are early maturing. The grain of ZS01452 has high test weight and an attractive, bright, orange color.

The foregoing is set forth by way of example and is not intended to limit the scope of the invention.

This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01452. Further, both first and second parent corn plants can come from the inbred corn line ZS01452. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01452 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like.

Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01452 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line ZS01452.

Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980's was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues.

European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982.

A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The deposit of at least 2500 seeds will be from the same inbred seed taken from the deposit maintained by ICI Seeds. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period.

Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office.

Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein. 

We claim:
 1. Inbred corn seed designated ZS01452, some of said seed deposited in the ATCC and carry accession number X204112.
 2. A corn plant produced by the seed of claim
 1. 3. A tissue culture of regenerable cells of ZS01452 of claim 1 wherein the cells of the tissue culture regenerate plants having the genotype of ZS01452.
 4. A tissue culture according to claim 3, the tissue culture selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof.
 5. A corn plant having the genotype of ZS01452 regenerated from the cells of the tissue culture of claim
 3. 6. Hybrid seed produced by:(a) planting, in pollinating proximity, seeds according to claim 1 of corn inbred lines ZS01452 and another inbred line, one of said inbred lines not releasing pollen; (b) cultivating corn plants resulting from said planting; (c) allowing natural cross pollinating to occur between said inbred lines; and (d) harvesting seeds produced on the non pollen releasing inbred.
 7. Hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01452 in claim 1 and plants of another inbred line.
 8. Hybrid plants grown from seed of claim
 7. 9. A first generation (F1) hybrid corn plant produced by using ZS01452 according to claim 1 the process of:(a) planting, in pollinating proximity, seeds of corn inbred lines ZS01452 and another inbred line; (b) cultivating corn plants resulting from said planting; (c) preventing pollen production by the plants of one of the inbred lines; (d) allowing natural cross pollinating to occur between said inbred lines; (e) harvesting seeds produced on plants of the inbred line of step (c); and (f) growing a harvested seed of step (e).
 10. A tissue culture of the regenerable cells of the corn plant of claim
 8. 11. A tissue culture of the regenerable cells of the corn plant of claim
 9. 